IMPACT OF ISATINE AND BROMISATINE ON IONIC CHANNELS OF MOLLUSKS
Shabanov P.D., Vislobokov A.I.
UDC: 612.8:615.7+576.3:594
Abstract: Changes in transmembrane calcium, sodium and potassium ionic currents during extracellular administration of heterocyclic compounds, isatine and bromisatine, in concentrations from 10-8 till 10-3 моl/l have ben studied. Method of intracellular dialysis and fixation of membrane potential in the mollusk Lymnaea stagnalis neurons have been used. Both compounds produced dose-dependant and reversible inhibitory action on neuron currents amplitude. It was - 5-35% from control indices in all intervals of concentrations, bromisatine inhibiting action being more significant. Kinetics of currents development do not change, but the shift of voltage-amper membrane characteristics to the right according to axis of potentials by 5-10 mV was registered. Therefore, both isatine and bromisatine possess membranotropic activity.
Key words: изатин, бромизатин, ионный ток, нейрон, моллюск, lymnaea stagnalis, isatine, bromisatine, ionic currents, neuron, mollusk, Lymnaea stagnalis
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MOLECULAR EPIDEMIOLOGY OF STAPHYLOCOCCUS AUREUS IN RUSSIAN PEDIATRIC HOSPITALS
Romanov A.V., Dekhnich A.V., Edelstein M.V.
UDC: 616.981.25-036.2:614.211-053.2+576.851.25
Abstract: The aim of investigation was to reveal molecular types of nosocomial and community-acquired strains of S. aureus in different regions of Russia. 575 strains, isolated from pediatric patients during multicenter studies were included in the study. For molecular typing 186 strains with unique resistance profile within each ward of each medical institution were selected. The following typing methods were used: MLVA, spa, SCCmec and MLST. According to MLVA, methicillin-susceptible strains were clustered to 80 types, that could be placed into 10 genetic groups that differ in not more than 1 VNTR locus. At the same time the vast majority of MRSA strains were represented by the only genetic group, consisted of 17 MLVA types. Only 2 types of SCCmec were detected (III and IV). According to MLST results the vast majority of strains belong to ST 239 and ST 8. Community-acquired MRSA had the same MLVA, spa and SCCmec types as nosocomial ones: t008 SCCmec IV, t037 SCCmec III and t030 SCCmec III. In our study, independently of the geographic location, place of onset of infection, and time period, the dominating molecular types of MRSA were ST8, t008, SCCmec IV and ST239, t030 and t037 SCCmec III.
Key words: MRSA, детские стационары, молекулярные методы типирования, MRSA, pediatric, molecular typing
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CLONAL DISSEMINATION OF CTX-M-5-PRODUCING NOSOCOMIAL STRAINS OF SALMONELLA TYPHIMURIUM IN RUSSIA, BELARUS, AND KAZAKHSTAN
Kozireva V.K., Edelstein M.V., Tapalsky D.V., Azizov I.S., Romanov A.V., Kozlov R.S.
UDC: 616.981.49-036.2+576.851.49
Abstract: In this paper, the evidence of broad geographic dissemination of Salmonella enterica serovar Typhimurium strains belonging to a single genetic lineage is presented. The isolates described possess resistance to oxiimino-cephalosporines due to production of CTX-M-5 extended-spectrum b-lactamase. The genetic relatedness of the strains isolated during multiple outbreaks of nosocomial salmonellosis in various parts of Russia, Belarus and Kazakhstan was established be pulsed-field gel electrophoresis (PFGE) and multi-locus tandem repeat analysis (MLVA). Circulation of the clone was first noticed in 1994 and is currently ongoing according to the data presented. The resistance to multiple antibiotics including penicillin-inhibitor combinations and various non-b-lactam drugs is mediated by large conjugative plasmids, while bla gene is associated with ISEcp1 element and carried by small non-conjugative plasmid. Some isolates express resistance to nalidixic acid owing to mutations in QRDR of gyrA gene.
Key words: Salmonella, Typhimurium, нозокомиальные инфекции, клональное распространение, антибиотикорезистентность, Salmonella, Typhimurium, nosocomial infection, clonal dissemination, antimicrobial resistance
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ACTION OF SELENIUM-CONTAINING METAL-COMPLEX SUBSTANCES ON RECTAL TEMPERATURE IN MICE IN PARENTERAL AND ENTERAL ADMINISTRATION
Sosin D.V., Yevseyev A.V., Parfenov E.A., Pravdivtsev V.A., Yevseyeva M.A., Kovaleva L.A.
UDC: 612.5:[546.23+612.273
Abstract: In experiments on mice influence of the new antihypoxants (selenium-containing metal-complex substances πQ1983, πQ2170) on rectal temperature level dynamic after parenteral and enteral introductions has been studied. It has been established that both substances perform clear dose-dependent hypothermic effect exceeding an action of the antihypoxant amthizole. It has been supposed that the substance πQ2170 has a high acute toxicity confirmed by a significant death-rate in mice.
Key words: мыши, антигипоксанты, ректальная температура, способы введения веществ, острая токсичность, mice, antihypoxants, rectal temperature, drug administration, acute toxicity
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THE INFLUENCE OF TIAZOLOINDOLE’S ANTIHYPOXANTS ON THE FUNCTIONAL STATE OF CENTRAL NERVOUS SYSTEM OF ANIMALS
Dikmanov V.V., Novikov V.E., Marysheva V.V.
UDC: 616-001.8:615.355
Abstract: With experiments on rats in tests «open field» and «elevated plus maze» it has been found that tiazoloindole derivatives under the codes VM-601 VM-606 VM-615 have a depressing effect on the functional state of the central nervous system. Compounds have a stress protection effect in hypoxia. Discovred properties of tiazoloindole derivatives probably have an impact in their antihypoxic effect.
Key words: гипоксия, производные тиазолоиндола, «открытое поле», «приподнятый крестообразный лабиринт», hypoxia, tiazoloindole derivatives, «open field», «elevated plus maze»
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EXPERT COMMISION AS AN ESSENTIAL FACTOR OF QUALITY CONTROL IN A CITY DENTAL OUT-PATIENT CLINIC
Shcherbakova T.E., Tsepov L.M.
UDC: 614.2:616.31-082-058
Abstract: The issues of multi-criteria quality evaluation of diagnostics and treatment of dental diseases depending on some quantitative and qualitative parameters are discussed in the paper. Quality examination allows optimizing the particular complex of diagnostic and treatment manipulations and ensuring patients’ right to proper quantity and quality of dental care.
Key words: комплексная оценка, диагностика, лечение, стоматологические заболевания, качество помощи, стоматологическая помощь, complete estimation, diagnostics, treatment, dental diseases, quality of dental care, dental care
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METHODS TO DECREASE BLOOD LOSS IN TOTAL HIP REPLACEMENT SURGERY
Petrov V.S., Obukhov V.A., Petrova M.M.
UDC: 616.728.2.-005.1+617.582-089.28-089.811
Abstract: In the article the problem of blood loss in orthopedic surgery is discussed. Application of tranexamic acid and a method of controlled hypotension lead to effective decrease of intra- and postoperative blood loss in total hip replacement surgery.
Key words: кровопотеря, транексамовая кислота, управляемая гипотония, трансплантация тазобедренного сустава, blood loss, tranexamic acid, method of controlled hypotension, replacements of a hip joint
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ELECTROENCEPHALOGRAPHIC CHARACTERISTICS IN CHILDREN WITH HEADACHE
Fetisova E.S., Yurieva N.V.
UDC: 616.857-053.2-073.7
Abstract: The most common form of headache in children as well as an attempt to identify the main EEG correlates has been studied.
Key words: головная боль, дети, электроэнцефалография
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UDC: 616.341-084-092
Key words: случай из практики, целиакия, глютеновая энтеропатия, наследственная энзимопатия, case report, coeliakia, gluten enteropathy, inherited enzymopathy
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GIRL HORMONAL ACTIVITY STATUS IN ONTOGENESIS
Fedorov G.N.
UDC: 612.621.3.018-053.2:611.013
Abstract: The article represents the analysis of the endocrine system function in young girls (2-16 years old) in different periods of ontogenesis. To provide an individual diagnostic approarch to assess the endocrine system condition certain studies to determine the level of hormones in the peripheral blood have been performed.
Key words: девочки, гормоны, онтогенез, girls, hormones, ontogenesis
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DEVELOPMENT AND POSSIBILITIES OF ENDOSCOPIC METHODS AND CHROMOENDOSCOPY IN DIAGNOSTICS OF UPPER GASTROINTESTINAL TRACT PATHOLOGY
Kostyakova E.A.
UDC: 616.3-072.1
Abstract: In this survey the main periods in endoscopic diagnostics of the upper gastrointestinal tract pathology (rigid, semi flexible, fiber optic and electronic) are highlighted. New endoscopic methods (chromo endoscopy, virtual chromo endoscopy) are described and their possibilities in detailed diagnostics of upper gastrointestinal tract pathology are characterized.
Key words: эндоскопия, хромоэндоскопия, желудочно-кишечный тракт, патология, endoscopy, chromo endoscopy, gastrointestinal tract, pathology
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CHARACTERISTICS OF OTONEUROLOGIC DISORDERS IN PATIENTS WITH DEMYELINATING DISEASES OF THE NERVOUS SYSTEM
Otvagin I.V., Maslova N.N., Kovaleva E.A.
UDC: 616.832-004.2:616.21
Abstract: The article contains the review of facts concerning characteristics of otoneurologic disorders in patients with demyelinating diseases of the central nervous system. Vestibular disorders are one of the most common signs of demyelinating diseases of the central nervous system.
Key words: рассеянный склероз, головокружение, нистагм, multiple sclerosis, dizziness, nystagmus
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ANALYSIS OF PHARMACOTHERAPY OF ALLERGIC DISEASES IN OUTPATIENT CLINICS (SMOLENSK)
Krikova A.V., Ostashuk M.S.
UDC: 616-056.3(470.332)+615.218.2
Abstract: The article presents the social and demographic portrait of patients with allergic disease. The study demonstrates thet it is necessary to improve the quality of treatment involving both doctors and pharmacists.
Key words: аллергические заболевания, антигистаминные препараты, амбулаторные пациенты, allergic diseases, antihistamine remedies, outpatients
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PALLIATIVE TREATMENT OF PATIENTS WITH DISSIMINATED RENAL CANCER
Zuj V.S., Solovyev V.I.
UDC: 616-006.04-08-039.75
Abstract: The aim of the study is to determine a method of choice in dissiminated renal cancer. Some experts deny positive influence of palliative surgical and other methods of influence on tumor process in kidney cancer. Some improvement in early diagnostics of the disease has taken place, but high indicators of IV stage of the process require further study of this problem.
Key words: рак почки, паллиативное лечение, kidney cancer, palliative care
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STRATEGICALLY AND TACTICAL OPTIMAL METHODS OF HEALTH-MANAGEMENT IN OCCASION OF PREVENTION AND REHABILITATION OF ALCOHOLIC DEPENDENCE PEOPLE IN THE REGION
Okhapkin A.S., Berks P.M., Kamanin E.I.
UDC: 612.46.23
Abstract: In this article we cover modern models of prevention and health rehabilitation of the population, suffering from alcoholic dependence.
Key words: реабилитация, профилактика, алкогольная зависимость, rehabilitation, prevention, alcoholic dependence
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«SPIRAL OF LIFE» (THE FIFTIETH ANNIVERSARY OF THE AWARDING OF FRANCIS CRICK, JAMES WATSON AND MAURICE WILKINS WITH THE NOBEL PRIZE «FOR THEIR DISCOVERIES CONCERNING THE MOLECULAR STRUCTURE OF NUCLEIC ACIDS AND THEIR SIGNIFICANCE FOR INFORMATION TRANSFER IN LIVING SYSTEMS»)
Korolev A.S., Litvinov A.V.
UDC: 61(09):575.1:576.1:577.1
Abstract: This year is the fiftieth anniversary since the time when the Noble committee made a decision to award Francis Crick, James Watson and Maurice Wilkins in the physiology and medical field «for their discoveries concerning the molecular structure of nucleic acids and their significance for information transfer in living systems».
Key words: геном, нуклеиновая кислота, живые системы, код, лаборатория, genome, nucleic acid, living systems, code, laboratory
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SOCIAL ISOLATION CHANGES ASYMMETRICALLY THE CONTENT OF MONOAMINES IN THE BALB/C MICE BRAIN
Karpova I.V., Mikheev V.V., Bychkov E.R., Lebedev A.A., Shabanov P.D.
UDC: 612.821.015.3+569.324.3
Abstract: The purpose of the study is to investigate the role of interhemispheric asymmetry of the dopaminergic and serotoninergic systems of the brain in formation of the syndrome of social isolation. The contents of dopamine (DA), serotonin (5-HT) and their metabolites dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindolacetic acid (5-HIAA) have been determined in the cortex and subcortical structures of both the right and the left hemispheres of the brain in the BALB/c mice reared in groups and social isolation. The isolated mice are characterized by reduced level of DA in the left striatum and elevated level of 5-HIAA and ratio 5-HIAA/5-HT in the right striatum. In the hippocampus of isolated mice, the activation of both DA-ergic and 5-HT-ergic systems was observed, that is the high level of DA and DOPAC in the left hippocampus and the elevated level of 5-HT in both hemispheres and of 5-HIAA in the right hippocampus were registered. On the other hand, the reduction of both DA-ergic and 5-HT-ergic systems activity was shown to be in the right hemisphere. Decreased concentration of DOPAC and ratio DOPAC/DA in the right cortex were observed as well. As to 5-HT-ergic system, the reduced level of 5-HT in the both cortex of the hemispheres as well as 5-HIAA in the right hemisphere of isolated mice has been determined. The phenomenon of interhemispheric asymmetry characterized by high DA-ergic activity in the left hippocampus compared to contralateral hemisphere is revealed only in the hippocampus but not in the striatum and the cortex though the different changes in DA and 5-HT metabolism in social isolation were registered.
Key words: межполушарная асимметрия, социальная изоляция, дофамин, серотонин, метаболизм, interhemispheric asymmetry, social isolation, dopamine, serotonin, metabolism
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